46 Adrenomedullin: A Novel Peptide Hormone for Uterine Receptivity and Conceptus Elongation in Pigs?
نویسندگان
چکیده
Abstract Adrenomedullin (ADM) is an evolutionarily conserved multi-functional peptide hormone in mammalian species. The implantation sites of conceptuses (embryo/fetus and its extra-embryonic membranes) pregnant Adm+/− female mice are abnormally spaced leading to fetal crowding, intrauterine growth restriction (IUGR) increases pregnancy loss. As litter-bearing species, pigs exhibit a high incidence early embryonic death (30-40%) naturally occurring IUGR (15-25%), significant numbers stillborn piglets (3-9%). Therefore, it imperative that the roles ADM regulation uterine receptivity, as well development conceptus be established. This study determined abundances luminal fluid, patterns expression receptor components uteri from cyclic gilts, during peri-implantation period pregnancy. include: calcitonin receptor-like (CALCRL; G protein-coupled bound by ADM), activity modifying protein (RAMP2; dimerized with CALCRL form ADM1 receptor) RAMP3 (dimerized ADM2 lower specificity ADM) atypical chemokine 3 (ACKR3; decoy serves cell-autonomous molecular rheostat dampen signaling). Total recoverable was greater fluid compared gilts between days 10 16 post-estrus epithelial (LE) trophectoderm (Tr) cells. Uterine CALCRL, RAMP2, ACKR3 were affected day (P < 0.05), status 0.01) and/or x 0.05). Within porcine conceptuses, RAMP2 increased Using established (pTr1) cell line isolated elongating recovered on 2 post-breeding, 10-7 M stimulated proliferation pTr1 cells 0.05) at 48 h, phosphorylated mechanistic target rapamycin (p-MTOR) 4E binding (p-4EBP1) 6.1- 4.9-fold 0.0001), respectively. These novel results indicate role for receptivity pigs. They also provide framework future studies signaling affect migration Tr cells, spacing blastocysts, placentation research supported Agriculture Food Research Initiative Competitive Grant no. 2022-67015-36491 USDA National Institute Agriculture.
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ژورنال
عنوان ژورنال: Journal of Animal Science
سال: 2023
ISSN: ['0021-8812', '1525-3163', '1525-3015', '1544-7847']
DOI: https://doi.org/10.1093/jas/skad068.049